Effects of Nigella sativa Seeds & Plantago ovata Husk on Fat Rich Diet Induced Inflammatory Responses in Rattus norvegicus

Consumption of herbal plants as a remedy to relieve the side effects of fat rich diets is on rise currently. The present study was conducted to scrutinize the impact of fat and fat reducing herbal agents on the serum proteins of Rattus norvegicus. Four groups of adult (A) & weaning (W) R. norvegicus one control and three experimental groups were designated as 0, I, II and III and were fed on rat chow, fat rich diet (FRD), FRD + 5% Nigella sativa seeds and FRD + 5% Plantago ovata husk respectively. The total serum protein concentrations in adult groups A-I & A-II were significantly elevated (P<0.01) while significantly lower concentrations were found in group A-III (P<0.001). The comparative serum profile of weaning groups revealed protein fractions of 201KDa in W-III while among the adult rat groups the protein fraction of 86 kDa in A-II group. The protein fractions of 295 KDa, 246 KDa, 133 KDa & 110 KDa were absent in group A-III as compared to other adult groups. It can be concluded that fat rich diets can alter the serum protein levels and seeds of N. sativa could preserve serum protein profile in adult R. norvegicus contrary to P. ovata husk which caused hypoproteinemia.